Permanent distal middle cerebral artery occlusion (PMCAO) stroke model is used to induce irreversible focal ischemia in rats, inducing a fronto-parietal cortical lesion sparing the subcortical structures. PMCAO can be induced in rats and mice by injection of the photothrombotic dye rose-bengal and irradiation of the brain with a cold light source leading to distal MCAO. The model leads to measurable histological damage and to predictable neurological deficits.
Avraham Y, Dayan M, Lassri V, Vorobiev L, Davidi N, Chernoguz D, Berry E, Leker RR (2012) Delayed leptin administration after stroke induces neurogenesis and angiogenesis. J Neurosci Res 91(2):187-195