Early (post weaning) isolation in rodents is associated with inceased anxiety and depressive like phenotypes, locomotor dysregulation in novel environments and increased stress vulnerability During the implementation of this model, immediately after weaning, animals will be housed individualy for 6 weeks in cages. Mice that will be group housed in cages of the same size will serve as controls. This procedure has been used extensively in both mice and rats.
Reproduced with permission from Elsevier
Ishikawa et al 2014: Effects of early social isolation (gray bars) and enriched environment (white bars-running wheel) on anxious like phenotypes demonstrated in adult rats undergoing the elevated plus maze test. Rats exposed to social isolation demonstrated more closed arms entries attesting to greater anxiety levels.
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