This model closely resembles the human disease. Pilocarpine is injected IP in divided doses, after pre-medication with lithium chloride and methyl-scopolamine. About 70% of the animals develop status epilepticus (SE) which is terminated after two hours with diazepam. Those animals will have chronic spontaneous seizures starting 4-8 weeks after SE.

References

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Glien M, Brandt C, Potschka H, Voigt H, Ebert U, Loscher W (2001) repeated low-dose treatment of rats with pilocarpine: low mortality but high proportion of rats developing epilepsy. Epilepsy Res 46(2):111-119

Mann A, Portnoy E, Han H, Inbar D, Blatch D, Shmuel M, Ben-Hur T, Eyal S, Ekstein D (2018). Folate homeostasis in epileptic rats.

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